In-vitro and ex-vivo experimental characterization of cardiac electrophysiology for:
- multiscale and heterocellular study of physiological and pathophysiological cardiac states
- characterization of temporal and spatial variability of cardiac electrophysiology in response to autonomic changes and metabolic states
- identification of structural and molecular determinants of arrhythmic risk in human ventricles
Technologies and targeting sectors:
- Cardiac biomarkers to characterize and predict heart dysfunction in relation to disease or metabolic states.
Targeting sector: Pharmaceutical and diagnostics companies. - Experimental tools to predict cardio-toxicity of drug compounds.
Targeting sector: Pharmaceutical companies - Discovery of novel cardiac therapies against pathophysiological states and metabolic diseases.
Targeting sector: Pharmaceutical and diagnostics companies.
Singular equipment and techniques:
- Optical Mapping (MiCAM O5-Ultima CMOS camera, SciMedia, Costa Mesa, CA) for electrophysiological recordings in cells and tissues
- Vibratome (VT12000s, LEICA) for the preparation of living myocardial tissue slices
- Mechanical electrostimulation system (C-Stretch and C-Pace M, Ion Optix) for organotypic tissue and cell culture
- Force transducer (Myotronic Heilderberg) for contractile force measurements
- Automatic Patch Clamp System (Q-patch Compact, Sophion, Denmark) for high-throughput electrophysiological recordings from ion channels in cells
- Microelectrode array system (MEA2100-Mini-60 System, Multichannel systems) for electrophysiological recordings in cells and tissues
- Tissue culture system with mechanical electrostimulation (MyoDish MD-1.2, In Vitro Sys) for biomimetic tissue culture with real time force monitoring
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